Metabolomics and proteomics

• Describe the general principles of sample preparation of small organic molecules and proteins, e.g. using solid phase extraction (SPE).
• Explain the general principle of chromatography (LC, GC) including the use of different relevant types of columns.
• Explain the general principles of diode array detection (DAD) and mass spectrometry (e.g. QTOF) coupled to liquid chromatography (LC-DAD-MS).
• Demonstrate the principles of dereplication and mass spectrometry.
• Perform data analysis using standard instrumental software for LC-DAD-MS analysis.
• Compare analytical results to information in commercial databases.
• Explain the basic principles behind quantitative analysis of small organic molecules.
• Explain the principles behind liquid chromatography and mass spectrometry based (e.g. Orbitrap and MALDI TOF) analysis of proteins and modified proteins.
• Describe common methodology in proteomics, interactomics, and post-tranlational modification omics-based workflows from different sample types.
• Understand and apply various ways of conducting quantitative proteomics analyses, e.g label-free vs. labeled, and Data-Dependent Acquisition vs. Data-Independent Acquisition.

Industrial production of small organic biomolecules (metabolites or natural products), proteins and modified proteins using microbial cell factories and mammalian cells, and cell products destined for cell therapies, is estimated to increase dramatically in the coming years. This includes important products such as antibiotics, anticancer agents, smaller proteins, and food ingredients like aroma compounds, pigments, antioxidants and vitamins. Other metabolites are toxic to humans and are therefore to be avoided in food and feed. Moreover, the need for improved understanding of cell behaviour at the molecular, and especially, protein level, has increased demands for improved proteomics workflows on ever decreasing sample amounts in a wide array of biological systems. This course will teach the basic principles behind modern methods for analysis of small organic molecules and proteins, especially based on liquid chromatography coupled to mass spectrometry. The topics will be presented in a mixture of lectures, exercises, group work with presentations.

26400/27022

Lectures, exercises, literature search, group work and student presentations

Minimum: 10.

Please be aware that this course will only be held if the required minimum number of participants is met. You will be informed 8 days before the start of the course, whether the course will be held.