Single-Course English 5 ECTS

Mammalian cell technology for manufacturing of biologics

Overall Course Objectives

The main objective is to give the students the necessary competences to master the requirements for, and principles of, cell line development, cultivation of mammalian cells for protein expression and production in the context of industrial biopharmaceutical production.

Learning Objectives

  • Evaluate the pros and cons of mammalian production cell lines and the relevant methods and strategies for expression of recombinant proteins in mammalian cells.
  • Describe the different types of products and the associated production challenges using mammalian hosts.
  • Explain the different steps of the cell line development process from expression construct to a monoclonal cell line.
  • Differentiate between the four phases of a cultivation of mammalian cells.
  • Discuss the implications of physiology and metabolism of mammalian cells in a suspension culture.
  • Determine different methods for medium optimization and process design for mammalian cell cultures.
  • Differentiate in detail the differences of post translational modifications made by mammalian cells relative to other eukaryotes.
  • Outline the use of mammalian cells in industrial reference processes.
  • Design processes for genetic manipulation and genome editing of mammalian cells with the purpose of producing recombinant proteins.
  • Discuss the application of genome-scale technologies (e.g. transcriptomics and proteomics) for engineering of mammalian cell cultures.
  • Describe methods for harvest, purification and characterization of protein products
  • Apply the theoretical learnings stated above in the analysis of production processes for pharmaceutical proteins and suggest ways of process optimization based on key process parameters.

Course Content

Biotechnology methods and techniques applied in the context of mammalian cell cultures.
A technical understanding of bioreactors for mammalian cell cultures.
Theoretical understanding of the mammalian cell cultivation and cell line development for production of biopharmaceuticals.
Protein purification, analysis for quality assessment of pharmaceutical proteins.
Cultivation physiology and process optimization.
The protein glycosylation (post translational modification) system in eukaryotes, specifically mammals.
Engineering of mammalian cells.
Regulatory aspects/intellectual property.
Recombinant therapeutic products: mAbs, vaccines, cell/gene therapies.

Recommended prerequisites

27026/27430/27022, Basic knowledge of molecular biology, cell biology and protein chemistry.

Teaching Method

Lectures and group work

Limited number of seats

Minimum: 10.

Please be aware that this course will only be held if the required minimum number of participants is met. You will be informed 8 days before the start of the course, whether the course will be held.

See course in the course database.

Registration

Language

English

Duration

13 weeks

Institute

Bioengineering

Place

DTU Lyngby Campus

Course code 27470
Course type Candidate
Semester start Week 5
Semester end Week 19
Days Fri 8-12
Price

7.500,00 DKK

Please note that this course has participants limitation. Read more

Registration