Course English

Introduction to Mammalian Bioprocessing

Cell Culture Engineering

This short course will give you a hands-on introduction to the production of biopharmaceutical proteins in mammalian cells. Through a mix of hands-on lab, theoretical exercises and lectures, the course will guide you through topics on mammalian cell cultivation, bioprocess modes, bioprocess control, cell line engineering, single-use equipment, and with a particular focus on perfusion bioprocess. Upon completion of the course, you will have sufficient overview, theoretical knowledge, and practical hands-on experience to e.g., get more company-specific training; better understand and support the upstream department; or to participate in management level decision-making on the covered topics.

In addition to extensive hands-on experience with the process, this course will also address many of the common concerns when implementing single-use processes – i.e., sterile connections, environmental concerns/carbon footprint, business economics, and validation.

Course schedule

Day 1

  • Lecture 1: Bioreactors: Purpose, history and types
  • Lab 1
    • Team 1: Thaw, TubeSpin, Glass 1L 
    • Team 2: 2L SU batch, fed batch and perfusion
  • Lecture 2: Single use vs stainless steel
  • Lab 2
    • Team 2: Thaw, TubeSpin, Glass 1L
    • Team 1: 2L SU batch, fed batch and perfusion

Day 2

  • Lecture 3: Bioprocess: Modes and concepts 
  • Lecture 4: Controls: CQA, CPP, PID, PAT 
  • Lab 3
    • Team 1: 2L SU batch, fed batch and perfusion
    • Team 2: Thaw, TubeSpin, Glass 1L
  • Lecture 5: Harvesting 
  • Lab 4:
    • Team 2: 2L SU batch, fed batch and perfusion
    • Team 1: Thaw, TubeSpin, Glass 1L

Day 3

  • Lab 5
    • Team 1: Wave and 50L
    • Team 2: Sampling and AMBR
  • Lecture 6: Perfusion 
  • Lab 6
    • Team 2: Sampling and AMBR
    • Team 1: Wave and 50L
  • Lecture 7: Industry Example

* The course schedule is subject to possible adjustments.

Learning outcomes

At the end of this course, participants should be able to:

  • Design of animal cell culture bioreactors, including suspension and immobilized cell bioreactors, conventional stainless steel stirred tanks, and disposable (single-use) bioreactors
  • Identify advantages and disadvantages of single-use technologies relative to multi-use equipment
  • Understand the pros and cons for bioreactor types (rocker, stirred tank, microcarrier) and strategies (batch, fed-batch, perfusion)
  • Setup and operation of single-use options for upstream manufacturing (rocker, stirred tank and perfusion)
  • Advanced automation and control of animal cell culture processes, including bioreactor Process Analytical Technology (PAT)
  • Basic cost calculations to compare single-use options to more traditional multi-use set ups, to provide justification for single-use technologies.

Who should attend?

This course is intended for participants who are:

  • Professionals within the biopharmaceutical industry who want to gain hands-on experience with mammalian bioprocessing equipment
  • Professionals who want to gain an understanding of upstream mammalian bioprocessing
  • Professionals working indirectly with mammalian bioprocessing who want to better understand it


Participants do not need to have specific knowledge in the field of biotechnology other than a basic high-school level understanding of natural sciences.

Important information 

Here you will find important information for short course participants regarding cancellation policies, location and waitlist policies.


To register for the course please click “Add to basket”.

Registration deadline is 2 weeks prior to course start.

In case, you have any questions related to your registration, please contact Khaled Chatila (

Discount for start-ups and academics

We offer employees in start-up companies, educational institutions and the public sector a 25% course fee reduction. Tick off the relevant boxes in the registration form to apply for this discount. You will get to the registration form when adding the course to the basket.

Companies wishing to arrange a tailored/private course are more than welcome to contact us to discuss this opportunity.

The AIM-Bio project – funded by the Novo Nordisk Foundation

This course was developed in the framework of the AIM-Bio project (Accelerated Innovation in Manufacturing Biologics), in collaboration between DTU Bioengineering, DTU Chemical Engineering, and the Biomanufacturing Training and Education Center (BTEC) at North Carolina State University. The AIM-Bio project is funded by the Novo Nordisk Foundation.

Course instructor



3 days


DTU Lyngby Campus


See available discounts at the bottom of the page


27.000,00 DKK