Hands-On Introduction to Preparative Chromatography

Day 1 (08:30-16:00)

• Lecture: Course orientation
• Lecture: From upstream to downstream: an overview
• Lecture and workshop: Resins and column packing
• Lecture and workshop: Classes of chromatography
• Lecture: Introduction to lab work
• Lab: Buffer Preparation and chromatography workstation software

Day 2 (08:30-16:00)

• Lab: Preparative ion exchange chromatography (IEX)
• Lab: Preparative affinity chromatography (IMAC)
• Lab: Data collection and discussion
• Lab: Analytical size exclusion chromatography (SEC) and gel electrophoresis (SDS-PAGE)
• Lecture: Scale-up (industry speaker)

Day 3 (09:00-13:30)

• Lab: Data analysis and system clean up
• Discussion
• Groupwork and presentations

* The course schedule is subject to minor possible adjustments.

At the end of this course, participants will be able to:

1. Gain a broad understanding of downstream processing and explain the principles and applications of multiple types of chromatography in protein purification.
2. Design a stepwise protein purification protocol based on physicochemical properties and process requirements.
3. Interpret chromatograms to evaluate purification efficiency, identify target peaks, and estimate yield and purity.
4. Troubleshoot common issues in chromatographic protein purification using systematic problem-solving approaches.
5. Evaluate the scalability of purification processes and understand relevant regulatory considerations in the pharmaceutical industry.

This course is broadly applicable for professionals in the biotechnology, pharmaceutical, and biopharma industries who are involved in the development, production, or quality control of biologics. It is especially relevant for:

• Scientists and technicians working with protein purification or chromatography
• Process engineers involved in downstream development or scale-up
• QA/QC and regulatory affairs professionals seeking insight into purification workflows
• Researchers and academic staff transitioning to industrial applications
• Early career professionals looking to build core competencies in downstream processing
• Experienced staff wanting to refresh and update their knowledge with current best practices

Whether you’re new to downstream processing or looking for upskilling opportunities, this course offers an essential, hands-on introduction to preparative chromatography.

Participants do not need to have specific knowledge in the field of biotechnology other than a basic high-school level understanding of natural sciences.

Here you will find important information for short course participants regarding cancellation policies, location and waitlist policies.

Physical (Lectures and group work)

To register for the course please click “Add to basket”.

The registration deadline is 1 week prior to course start.

In case, you have any questions related to your registration, please contact Course Coordinator Khaled Chatila (khachat@dtu.dk)

The course will take place at the Section for Biologics Engineering, DTU Bioengineering on DTU’s Lyngby campus

We offer employees in start-up companies, educational institutions and the public sector a 25% course fee reduction.

Tick off the relevant boxes in the registration form to apply for this discount. You will get to the registration form when adding the course to the basket.

Tailor-made format for companies

Companies wishing to arrange a tailored/private course are welcome to contact us to discuss this opportunity.