Instrumental Chemical Analysis

• Describe the principles of operation of the instruments, HPLC, GC, MS, AAS and ICP.
• Describe the principle components of MS instruments.
• From a list of molecules decide which instruments are well suited for identification and analysis of these molecules.
• Identify the type of samples that may be applied to specific instruments.
• List the advantages and drawbacks of the analytical protocols (LC, GC, MS and biosensors).
• Propose a method of sample preparation that is required for the analytical method .
• Describe the basic principles of separation on columns for HPLC.
• Describe the basic principles of separation by Size Exclusion Chromatography (SEC) and how different detector combinations and calibrations can be applied for polymer analysis.
• Give basic insight in validation and quality assurance.

Virtually every chemist and biochemist works with various analytical methods that are applied to production, quality control and research. Many important decisions in society are executed on the basis of results of chemical analysis. Examples comprise additives to food products, drinking water, waste water, quality of pharmaceuticals, nutrients and forensics. Process control constitute another areas of research, where chemical analysis and biochemical analysis are important.
A wide variety of technologies are treated at the course, such as sample preparation, spectrometric technologies and automatic technologies. A introduction is given to chromatography comprising gas chromatography (GC) and high performance liquid chromatography (HPLC). The separation technologies are hyphenated with detectors, such as mass spectrometers, photo multipliers (diode array), electrodes and light scattering. Measurements by other spectrometric methods and analysis by biosensors also has widespread application.

Inorganic chemistry:
Electroanalysis (ISE, coulometry, stripping analysis)
Optical methods (spectrometry. turbidimetry, AAS and ICP-AES).
Automatic methods.
Organic chemistry:
Methods for the separation and characterization of organic molecules including characterization of polymers.
Also LC-MS and ICP-MS.

• 6 – 20 (Thurs 8-12)

26300/26428/27022, The basic course of chemistry or biotechnology must be passed.

Lectures and exercises

Minimum: 4.

Please be aware that this course will only be held if the required minimum number of participants is met. You will be informed 8 days before the start of the course, whether the course will be held.