Course English 2.5 ECTS

Introduction to Chemical Modification of Protein Therapeutics

Introduction to Chemical Modification of Therapeutic Proteins

Want to gain an introduction to the chemical modification of protein therapeutics?

This course aims to cover an introduction to the chemical modification of protein therapeutics. This is an advanced technique of great importance to the future production of therapeutic proteins. Therefore, the modification of these proteins will be placed in the context of the current biopharmaceutical industry.

Course content:

1. Introduction to chemical modification of protein therapeutics in the context of drug development, including the benefits of such modifications. Techniques will include acylation, PEGylation and antibody-drug conjugation.

2. Processes for the production of chemically modified proteins, including reactor selection. Background chemistry to include sufhydryl and amino group chemistry.

3. Downstream processing, including impurity removal and product isolation, also via process chromatography (hydrophobic interaction, size exclusion and ion exchange).

For more details on scope, form and exam, please see course base.

Learning outcomes

By the end of this course a participant should be able to:

  • Describe chemical modification techniques relevant to protein therapeutics and their benefits, including acylation, PEGylation, and antibody-drug conjugation
  • Understand appropriate procedures to perform basic protein modifications, including reactor types and operation
  • List reaction parameters that must be controlled for successful modification
  • Describe the purification steps following modification required to remove residual reaction components and isolate the target, including process chromatography
  • Understand the approaches to analysis, such that modifications can be carefully controlled.
  • Understand the potential of new methods of chemical modification, such as enzymatic methods.

The course will be a mix of lectures, workshops and written exercises.

Who should attend?

The course is broadly applicable to anyone who is interested in learning about large-scale biomanufacturing operations and gaining hands-on experience in a simulated cGMP environment.

This course is perfect for:

  • New employees
  • Support personnel from Quality Control and Quality Assurance
  • Vendors and suppliers who provide equipment and services to the industry.

Prerequisites 

Some basic knowledge of MATLAB or Python is an advantage, but not a must.

Important information 

Here you will find important information for short course participants regarding cancellation policies, location and waitlist policies.

Fee, registration and location 

  1. This course is free for PhD students registered at a Danish university
  2. The course fee for PhDs registered at international institutions and faculty/staff employed by an academic institution is 7,000 DKK
  3. The course fee for industry participants is 10,000 DKK.

To register, please reach out to Program Coordinator, Seungmi Nam (seunam@dtu.dk). Send your name, university or company and department.

The application deadline is May 9th 2023.

The course will take place in the pilot plant facilities at the Department of Chemical and Biochemical Engineering on DTU’s Lyngby campus.

The AIM-Bio project – funded by the Novo Nordisk Foundation

This course was developed in the framework of the AIM-Bio project (Accelerated Innovation in Manufacturing Biologics), in collaboration between DTU Bioengineering, DTU Chemical Engineering, and the Biomanufacturing Training and Education Center (BTEC) at North Carolina State University. The AIM-Bio project is funded by the Novo Nordisk Foundation.

Course instructor

John M. Woodley

Professor and Bioprocess Engineer

Prof. John M. Woodley is a Bioprocess Engineer who has had an interest in the biological synthesis of useful chemicals, using biocatalysis and fermentation for that last 30 years. His research is at the interface of bioprocess engineering, process chemistry and reaction engineering. It includes both theoretical and experimental work at different scales:

– Multi-step biocatalysis, including systems biocatalysis and flow chemistry

– Downstream processing from biocatalytic reactors and fermentations, including ISPR

– Modelling tools for the assessment of bioprocesses, including thermodynamics, kinetics, process simulation, economic evaluation

– Bio-oxidations, including oxygen supply methods

Enzymes currently under investigation include alcohol oxidases, carbohydrate oxidases, cytochrome P450s, Baeyer-Villiger monooxygenases and transaminases.

Potræt af Bio-ingeniør John M. Woodley i blå skjorte og med grå baggrund

Registration

Duration

15-16 May 2023
Place

In-person on DTU Campus in Lyngby
ECTS

2.5

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