Course English

Cell Culture Engineering: A Single-Use Perspective

Cell Culture Engineering

Curious about production of biotherapeutics and growth of mammalian cells in bioreactors? Want to learn the fundamentals and actually try out the equipment?

Then sign up for this course, where you will get an introduction to mammalian cell culture, several types of bioprocess equipment, cell banking, scale-up and down and single-use bioreactors versus stainless steel bioreactors.

Get hands-on experience with bioprocess equipment, from 15mL to 50L, and a variety of single-use biomanufacturing options e.g., AMBR15, rocker bags, stirred tank and perfusion.

In addition to extensive hands-on experience with the process equipment, this course will also address topics and concerns common to implementing single-use processes – e.g., environmental concerns/carbon footprint and the economics of single-use and continues processes.

Please note, that we offer a discounts for start-ups and academics – see below.

Learning outcomes

At the end of this course, you should be able to:

  • Discuss differences between a variety of animal cell culture bioreactors, conventional stainless steel stirred tanks, and disposable (single-use) bioreactors
  • Identify advantages and disadvantages of single-use technologies relative to multi-use equipment
  • Understand the pros and cons for bioreactor types (rocker, stirred tank, microcarrier) and strategies (batch, fed-batch, perfusion)
  • Setup and operate single-use options for upstream manufacturing (rocker, stirred tank and perfusion)
  • Have a basic understanding of advanced automation and control of animal cell culture processes, including bioreactor PAT
  • Discuss basic cost calculations in regards to single-use options vs multi-use set ups, and to provide justification for single-use technologies.

Who should attend?

This course is intended for people who would:

  • Like to gain an introduction to both the practical and theoretical aspects of mammalian bioprocess work
  • Be working adjacent or managing groups performing mammalian bioprocess work
  • Like hands-on introduction to the equipment types.


This course has no specific prerequisites. It is an introductory course with a focus on hands-on training.

Important information 

Here you will find important information for short course participants regarding cancellation policies, location and waitlist policies.

Fee and registration 

Fee: Price to be confirmed.

To register for the course please click “Add to basket”.

The application deadline is August 31st 2023.

In case, you have any questions related to your registration, please contact Course Coordinator Husein Suta on

Discount for start-ups and academics

We offer employees in start-up companies, educational institutions and the public sector a 25% course fee reduction. Tick off the relevant boxes in the registration form to apply for this discount. You will get to the registration form when adding the course to the basket.

Companies wishing to arrange a tailored/private course are more than welcome to contact us to discuss this opportunity.

The AIM-Bio project – funded by the Novo Nordisk Foundation

This course was developed in the framework of the AIM-Bio project (Accelerated Innovation in Manufacturing Biologics), in collaboration between DTU Bioengineering, DTU Chemical Engineering, and the Biomanufacturing Training and Education Center (BTEC) at North Carolina State University. The AIM-Bio project is funded by the Novo Nordisk Foundation.

Course instructor



4-6 September 2023 from 8:30-16:30


In-person on DTU Campus in Lyngby


See available discounts at the bottom of the page


24.000,00 kr.

0 spots left